SamClipIndelFraction

Last commit

Extract clipping/indel fraction from BAM

DEPRECATED

This tool can be replace with Bioalcidaejdk

Usage

This program is now part of the main jvarkit tool. See jvarkit for compiling.

Usage: java -jar dist/jvarkit.jar samclipindelfraction  [options] Files

Usage: samclipindelfraction [options] Files
  Options:
    -h, --help
      print help and exit
    --helpFormat
      What kind of help. One of [usage,markdown,xml].
    -o, --output
      Output file. Optional . Default: stdout
    -R, --reference
      For reading/writing CRAM files. Indexed fasta Reference file. This file 
      must be indexed with samtools faidx and with picard/gatk 
      CreateSequenceDictionary or samtools dict
    --version
      print version and exit

Keywords

sam
bam
clip

Creation Date

20141118

Source code

https://github.com/lindenb/jvarkit/tree/master/src/main/java/com/github/lindenb/jvarkit/tools/misc/SamClipIndelFraction.java

Unit Tests

https://github.com/lindenb/jvarkit/tree/master/src/test/java/com/github/lindenb/jvarkit/tools/misc/SamClipIndelFractionTest.java

Contribute

Issue Tracker: http://github.com/lindenb/jvarkit/issues
Source Code: http://github.com/lindenb/jvarkit

License

The project is licensed under the MIT license.

Citing

Should you cite samclipindelfraction ? https://github.com/mr-c/shouldacite/blob/master/should-I-cite-this-software.md

The current reference is:

http://dx.doi.org/10.6084/m9.figshare.1425030

Lindenbaum, Pierre (2015): JVarkit: java-based utilities for Bioinformatics. figshare. http://dx.doi.org/10.6084/m9.figshare.1425030

Example

$ samtools view -h -F3844 my.bam  | java -jar dist/jvarkit.jar samclipindelfraction

##UNMAPPED_READS=0
##MAPPED_READS=3028359
##CLIPPED_READS=1182730
##CLIPPED_READS_5_PRIME=597757
##CLIPPED_READS_3_PRIME=617399
##UNCLIPPED_READS=1845629
##COUNT_BASES=338644685
#CLIP   COUNT   FRACTION_OF_MAPPED_READS
0   1845629 0.5
1   7   1.8963724562195327E-6
2   6756    0.0018302703306027376
3   695 1.8828269386751074E-4
4   794 2.1510281860547272E-4
5   819 2.2187557737768533E-4
6   471 1.275987752684857E-4
7   447 1.210969268471616E-4
(...)